The Lab is dedicated to the development of innovative materials, systems and methodologies for the study of Extracellular Vesicles (EVs).

We work on novel technological breakthroughs to accelerate the transition of EV based research to real clinical utility. Current projects are aimed at the implementation of:

  • Multimodal analytical platforms for EV characterization
  • Methods for affinity capturing and isolation of EVs in a scalable format

We are among the founders of EVIta: the Italian Society for Extracellular Vesicles

EVs counting and sizing
EVs microarrays for biomarkers analysis and discovery
Technologies for EVs isolation and characterization
EVs-based liquid biopsy

Featured publications

Digital detection of biomarkers assisted by nanoparticles: application to diagnostics

Marina Cretich, George G. Daaboul, Laura Sola, M. Selim U¨nlu¨ , and Marcella Chiari

Trends in Biotechnology  2015,  33,  6, 343-350

DOI: 10.1002/anie.201409678

Comparing Digital Detection Platforms in High Sensitivity Immune-phenotyping of Extracellular Vesicles

Roberto  Frigerio, Angelo  Musicò, …. Alessandro Gori, Marina Cretich

Journal of Extracellular Biology, Volume1, Issue8

doi.org/10.1002/jex2.53

Digital Detection of Exosomes by Interferometric Imaging

G. Daaboul, P. Gagni, L. Benussi, P.Bettotti, M. Ciani, M. Cretich, D. Freedman, R. Ghidoni, A. Yalcin Ozkumur, C.Piotto, D. Prosperi, B.Santini, M. Selim Ünlü and M. Chiari

Scientific Reports (2016) 6:  3746

DOI: 10.1039/C8CC06010J

Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis

A. Gori, A. Romanato, G. Bergamaschi, A. Strada, P. Gagni, R. Frigerio, D. Brambilla, R. Vago, S. Galbiati, S. Picciolini, M. Bedoni, G. G. Daaboul, M. Chiari, M. Cretich

Journal of Extracellular Vesicles 

DOI:

10.1080/20013078.2020.1751428

Core technologies and instrumentations

  • Counting and sizing of particles by Nanoparticle Tracking Analysis (NTA) using the Nanosight NS300
  • Western Blotting workflow and ChemiDoc Imager
  • High-sensitivity fluorescence custom-made microarrays for EVs phenotyping (EV arrays)
  • EVs counting, sizing an phenotyping through fluorescence co-localization by custom-made microarrays and ExoView platform
High sensitivity EV phenotyping by Quanterix SR-X Single Molecule detection system (SiMoa)

Projects

INDEX: Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes

H2020 FETOPEN — Grant agreement n°766466

http://www.indexproject.eu

HYDROGEX: A Peptide Hydrogel Platform for Extracellular Vesicles Isolation and Multimodal Analysis

Bando Materiali Avanzati. Fondazione Cariplo & Regione Lombardia; Grant n° 2018-1720

MARVEL: Evolving reversible iMmunocapture by membrane sensing peptides: towARds scalable extracellular VEsicLes isolation

H2020 FET PROACT – Grant Agreement n° 951768

MARVEL website